Anti-BOB1/POU2AF1 Antibody Picoband® fluoro594 Vervoeging

POU2AF1 (POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V (D) J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by ly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.

POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; BOB1; OCA-B; OCT-binding factor 1; Pou2af1; Obf-1

POU2AF1

18985

Q64693

Rat, Mouse

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1, different from the related rat sequence by one amino acid, and from human sequence by four amino acids.

B-cell specific.

Flow Cytometry

Adaptive Immunity, B Cells, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Immunology, Non-CD, Transcription

Immunogen affinity purified.

Liquid

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

1. Casellas, R., Jankovic, M., Meyer, G., Gazumyan, A., Luo, Y., Roeder, R. G., Nussenzweig, M. C.OcaB is required for normal transcription and V (D) J recombination of a subset of immunoglobulin kappa genes.Cell 110: 575-585, 2002. 2. Siegel, R., Kim, U., Patke, A., Yu, X., Ren, X., Tarakhovsky, A., Roeder, R. G.Nontranscriptional regulation of SYK by the coactivator OCA-B is required at multiple stages of B cell development.Cell 125: 761-774, 2006.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

27692 MW

No cross reactivity with other proteins.

6

POU domain class 2-associating factor 1

Nucleus.

POU domain class 2-associating factor 1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.