Anti-Brd4 Konijn Monoklonale Antilichaam

Boster Bio Anti-Brd4 Rabbit Monoclonal Antibody catalog # M00123. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Bromodomain-containing protein 4; Protein HUNK1; BRD4; HUNK1

BRD4

23476

O60885

Human, Mouse, Rat

A synthesized peptide derived from human Brd4

Clone: DBH-2

Ubiquitously expressed. .

WB, IHC, ICC, IF, IP

Cancer, Chromatin Binding Proteins, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, Transcription, Tumor Suppressors

Affinity-chromatography

0.5mg/ml

Liquid

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P- TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P- TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, ly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028) . In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters. .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M00123

152 kDa

70 kDa

Bromodomain-containing protein 4

Nucleus. Chromosome. Associates with acetylated chromatin. Released from chromatin upon deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or nocodazole treatment.

P2X purinoceptor 1

Rabbit IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.