Anti-BrdU APC

Bromodexyuridine (BrdU) is a thymidine analog, which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.

Bromodeoxyuridine conjugated to BSA

BrdU

Bu20a

FC-IC (QC tested)

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem. 1989 Oct;37 (10) :1517-27., URL: https://www.ncbi.nlm.nih.gov/pubmed/2476478, *O'Driscoll M, Jeggo PA: CsA can induce DNA double-strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair. Bone Marrow Transplant. 2008 Jun;41 (11) :983-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18278071, *Pajalunga D, Mazzola A, Salzano AM, Biferi MG, De Luca G, Crescenzi M: Critical requirement for cell cycle inhibitors in sustaining nonproliferative states. J Cell Biol. 2007 Mar 12;176 (6) :807-18., URL: https://www.ncbi.nlm.nih.gov/pubmed/17353358, *Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB: Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67 (MIB-5) immunohistochemistry and in situ hybridization for histone mRNA. J Histochem Cytochem. 2003 Dec;51 (12) :1681-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/14623936, *Lagares A, Li HY, Zhou XF, Avendaño C: Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. J Neurosci. 2007 Jul 25;27 (30) :7939-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/17652585, *Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF: Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'-bromo-2'-deoxyuridine. Am J Physiol Cell Physiol. 2005 Sep;289 (3) :C757-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/15857903, *Martín CA, Surur JM, García MN, Corrons F, Badrán AF: Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver. Biocell. 2005 Apr;29 (1) :33-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15954465, *Yatera K, Hsieh J, Hogg JC, Tranfield E, Suzuki H, Shih CH, Behzad AR, Vincent R, van Eeden SF: Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques. Am J Physiol Heart Circ Physiol. 2008 Feb;294 (2) :H944-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/18083905, *Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem. 1989 Oct;37 (10) :1517-27., URL: http://www.ncbi.nlm.nih.gov/pubmed/2476478

*Ashby J, Tinwell H, Soames A, Foster J: Induction of hyperplasia and increased DNA content in the uterus of immature rats exposed to coumestrol. Environ Health Perspect. 1999 Oct;107 (10) :819-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/10504149?dopt=, *Soames AR, Lavender D, Foster JR, Williams SM, Wheeldon EB: Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S-phase in rat and mouse liver. J Histochem Cytochem. 1994 Jul;42 (7) :939-44. , URL: https://www.ncbi.nlm.nih.gov/pubmed/8014477?dopt=A

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.

6

Mouse IgG1