Anti-Bub3 Antibody Picoband® fluoro488 Vervoeging

BUB3 (BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC) ; though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through ion of kinetochore localization during prometaphase to achieve biorentation. Bub3 s the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.

Mitotic checkpoint protein BUB3; BUB3

BUB3

9184

O43684

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human Bub3, identical to the related rat and mouse sequences.

Flow Cytometry

Cell Biology, Cell Cycle, Cell Division, Spindle

Immunogen affinity purified.

Liquid

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. .

1. Cahill, D. P., da Costa, L. T., Carson-Walter, E. B., Kinzler, K. W., Vogelstein, B., Lengauer, C. Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58: 181-187, 1999. 2. Kalitsis, P., Earle, E., Fowler, K. J., Choo, K. H. A. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev. 14: 2277-2282, 2000. 3. Morgan, David O (2007) . The cell cycle: principles of control. London: Published by New Science Press in association with Oxford University Press.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

37155 MW

No cross reactivity with other proteins.

6

Mitotic checkpoint protein BUB3

Nucleus. Chromosome, centromere, kinetochore . Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition. .

Belongs to the WD repeat BUB3 family.

Mitotic checkpoint protein BUB3

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.