Anti-BUB3 Antibody Picoband® fluoro594 Vervoeging

BUB3 (BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC) ; though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through ion of kinetochore localization during prometaphase to achieve biorentation. Bub3 s the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.

Eosinophil cationic protein; ECP; Ribonuclease 3; RNase 3; RNASE3; ECP; RNS3

BUB3

9184

O43684

Human, Rat

No cross-reactivity with other proteins.

E.coli-derived human BUB3 recombinant protein (Position: R50-D209) .

Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level) .

Flow Cytometry

2339, DNA/RNA, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, RNA Processing

Immunogen affinity purified.

Liquid

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

1. Cahill, D. P., da Costa, L. T., Carson-Walter, E. B., Kinzler, K. W., Vogelstein, B., Lengauer, C. Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58: 181-187, 1999. 2. Kalitsis, P., Earle, E., Fowler, K. J., Choo, K. H. A. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev. 14: 2277-2282, 2000. 3. Morgan, David O (2007) . The cell cycle: principles of control. London: Published by New Science Press in association with Oxford University Press.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

64406 MW

6

Ribonuclease A family member 3

Secreted. Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.

Transcription factor E2F3

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.