Anti-C-Raf (N-terminaal gebied) Antilichaam

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3) . The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259) /B-Raf (S365) ) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation

Our C-Raf (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat C-Raf (N-terminal region) and is protein g purified. It is great for use in ELISA, WB.

Our C-Raf (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat C-Raf (N-terminal region) and is Protein G purified. It is great for use in WB.

Raf1, CRaf

RAF1

P04049

Human, Mouse, and Rat

Clone (M208) was generated from a sequence corresponding to amino acids in the N-terminal region of human C-Raf. This C-Raf sequence has high homology to rat and mouse C-Raf.

C-Raf (N-terminal region)

M208

Unconjugated

ELISA, WB

Apoptosis, Cell Signaling, Kinase Signaling, Oncoproteins

Protein G Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

74

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein G purified culture supernatant.

1:1000

This antibody detects a 74 kDa* protein corresponding to the molecular mass of C-Raf on SDS-PAGE immunoblots of human A431 and Jurkat cells.

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_2175515