Anti-c-Src (Tyr-530) [beserveerde site], Fosfospecifiek antilichaam

C-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.

Our c-Src (Tyr-530) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat c-Src (Tyr-530) and is protein a purified. It is great for use in ELISA, WB.

Our c-Src (Tyr-530) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat c-Src (Tyr-530) and is Protein A purified. It is great for use in WB.

Src

SRC

P12931

Tyr-530

Human, Mouse, and Rat

Clone (M261) was generated from a phosphopeptide containing amino acid surrounding Tyr-530 at the C-terminus of human c-Src. This sequence has high homology to the conserved site in rat and mouse c-Src, and is conserved in the c-Src family of kinases.

C-Src (Tyr-530)

M261

Unconjugated

ELISA, WB

Cell Signaling, Kinase Signaling, Oncoproteins

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

60

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:500

This antibody detects a 60 kDa* protein corresponding to c-Src on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and treated with pervanadate. This antibody also detects the conserved site in Lck (Tyr-505) in human Jurkat cells treated with pervanadate. This antibody may detect several c-Src family members phosphorylated at the conserved site for Tyr-530.

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_2196030