Anti-CAD Antibody Picoband® 31.0550 Vervoeging

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

CAD protein; Glutamine-dependent carbamoyl-phosphate synthase

CAD

790

P27708

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human CAD recombinant protein (Position: R1403-D2198) .

Flow Cytometry

Apoptosis, Cancer, Cell Biology, Cell Cycle, Cell Death, DNA/Nucleotides, DNA/RNA, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Nuclear, Nucleotide Metabolism, Nucleus, Pathways and Processes

Immunogen affinity purified.

Liquid

This protein is a fusion protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase) .

1. Ben-Sahra, I., Howell, J. J., Asara, J. M., Manning, B. D. Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1. Science 339: 1323-1328, 2013. 2. Bertoni, L., Attolini, C., Simi, S., Giulotto, E. Localization of the Chinese hamster CAD gene reveals homology between human chromosome 2p and Chinese hamster 7q. Genomics 16: 779-781, 1993. 3. Chen, K.-C., Vannais, D. B., Jones, C., Patterson, D., Davidson, J. N. Chromosomal localization of the human CAD gene to 2p21-22. (Abstract) Am. J. Hum. Genet. 41: A161 only, 1987.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase

Cytoplasm.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.