Anti-Calpain 3/CAPN3 Antibody Picoband® fluoro647 Vervoeging

Calpain-3 is a protein that in humans is encoded by the CAPN3 gene. Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed.

Calpain 3/CAPN3

825

P20807

Human, Mouse

E.coli-derived human Calpain 3/CAPN3 recombinant protein (Position: I42-D764) . Human CAPN3 shares 93.5% and 94.2% amino acid (aa) sequence identity with mouse and rat CAPN3, respectively.

Flow Cytometry

Immunogen affinity purified.

Liquid

1. Blazquez, L., Azpitarte, M., Saenz, A., Goicoechea, M., Otaegui, D., Ferrer, X., Illa, I., Gutierrez-Rivas, E., Vilchez, J. J., Lopez de Munain, A. Characterization of novel CAPN3 isoforms in white blood cells: an alternative approach for limb-girdle muscular dystrophy 2A diagnosis. Neurogenetics 9: 173-182, 2008. 2. Canki-Klain, N., Milic, A., Kovac, B., Trlaja, A., Grgicevic, D., Zurak, N., Fardeau, M., Leturcq, F., Kaplan, J.-C., Urtizberea, J. A., Politano, L., Piluso, G., Feingold, J. Prevalence of the 550delA mutation in calpainopathy (LGMD 2A) in Croatia. Am. J. Med. Genet. 125A: 152-156, 2004. Note: Erratum: Am. J. Med. Genet. 130A: 218 only, 2004. 3. Duno, M., Sveen, M.-L., Schwartz, M., Vissing, J. cDNA analyses of CAPN3 enhances mutation detection and reveals a low prevalence of LGMD2A patients in Denmark. Europ. J. Hum. Genet. 16: 935-940, 2008.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

99 kDa

6

IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.