Anti-β-Catenin Antilichaam

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

Our β-Catenin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat β-Catenin and is protein a purified. It is great for use in ELISA, WB, ICC, IHC, IP.

Our β-Catenin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat β-Catenin and is Protein A purified. It is great for use in WB, ICC, IHC, IP.

Catenin beta1, CTNNB1, catenin

CTNNB1

P35222

Human, Mouse, and Rat

Clone (M118) was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin.

β-Catenin

M118

Unconjugated

ELISA, WB, ICC, IHC, IP

Cell Adhesion Molecules, Cell Signaling, Wnt Signaling, Immunology, Oncoproteins, Transcription Regulation

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

GOmAb

92

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:500

1:1000

1:100

The antibody detects a 92 kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells. In addition, this antibody recognizes only β-Catenin in immunoprecipitations using anti-β-Catenin versus γ-Catenin.

1:250

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_3068393