Anti-CD14 (Monocyte / Macrofage Marker) Monoklonale Antilichaam (Kloon: LPSR/2386)

Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor) . CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Lipopolysaccharide receptor; LPS-R; LPSR; Mo2; Monocyte Differentiation Antigen 14; Myeloid cell-specific leucine-rich glycoprotein

CD14

929

P08571

Human

Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)

LPSR/2386

WB, FACS, IHC

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Western Blot (1-2ug/ml) ; Flow Cytometry (1-2ug/million cells) ; , Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Mouse IgG1, kappa