Anti-CD33 / SIGLEC3 (Myelode Cell Surface Antigen) Monoklonale Antilichaam (Kloon: WM53) -CF488

Recognizes a 67kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505) . CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM) -containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Gp67; My9; Myeloid cell surface antigen CD33; p67; Sialic acid-binding Ig-like lectin 3 (SIGLEC3)

CD33

945

P20138

Human

Human AML cells

WM53

FACS, IF

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjμgated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous.

Flow Cytometry: 5ul per test per one million cells (or 5ul per 100ul of whole blood) ; Immunofluorescence (1:50-1:100)

Mouse IgG1, kappa

CF488