Anti-CD95 / FAS / TNFRSF6 Monoklonale Antilichaam (Kloon: B-R18)

MAb B-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro) . CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.

ALPS1A, Apoptosis APO1 antigen 1, Apoptosis-mediating surface antigen FAS, APT1, Delta Fas, , Fas AMA, Fas cell surface death receptor, FAS1, FASL receptor, FASLG receptor, FASTM, Surface antigen APO1, Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)

FAS

355

P25445

Human

Recombinant human CD95 protein

B-R18

Functional Assay, FACS, IF, IHC

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Functional Studies (Order Ab without Azide) ; , Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; , Immunohistochemistry (Frozen) (1-2ug/ml for 30 minutes at RT)

Mouse IgG1, kappa