Anti-Cdk5 Antilichaam

Cyclin-dependent kinases (Cdks) are a family of serine/threonine kinases that require association with regulatory subunits known as cyclins for activation. In addition, post-translational phosphorylation and dephosphorylation events regulate Cdk activity. Phosphorylation of Thr-160 in the T loop by Cdk-activating kinase (CAK) is an obligatory step in kinase activation. By contrast, phosphorylation of the Thr-14 and Tyr-15 residues by the Wee1 family of dual specificity kinases is inhibitory for the Cdks, and dephosphorylation of these residues by the Cdc25 family of phosphatases coincides with Cdk activation. Alternatively, Cdk5 appears to require different mechanisms for activation. This Cdk is activated through association with specific activators, including p35, p39, and p67. Cdk5 is primarily activated in neuronal cells, and only c-Abl kinase, rather than Wee family members, have been shown to phosphorylate Tyr-15 to regulate its activity.

Our Cdk5 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Cdk5 and is protein a purified. It is great for use in ELISA, WB, IP, ICC.

Our Cdk5 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Cdk5 and is Protein A purified. It is great for use in WB, IP, ICC.

PSSALRE, TPKII

CDK5

Q00535

Human, Mouse, and Rat

Clones DC17+DC34 were generated from a recombinant full-length human Cdk5 sequence.

Cdk5

DC17+DC34

Unconjugated

ELISA, WB, IP, ICC

Cell Signaling, Kinase Signaling, Oncoproteins

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

31

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:1000

1:100

The antibody detects a 31 kDa* band corresponding to Cdk5 on SDS-PAGE immunoblots of human SYF and HeLa cells, and in mouse brain tissue. The antibody does not detect other Cdk family members, such as Cdk1 and Cdk2.

1:100

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_2078862