Anti-CEP250 Antibody Picoband®

Centrosome-associated protein CEP250 is a protein that in humans is encoded by the CEP250 gene. This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Boster Bio Anti-CEP250 Antibody Picoband® catalog # A07150-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Gastrotropin; GT; Fatty acid-binding protein 6; Ileal lipid-binding protein; ILBP; Intestinal 15 kDa protein; I-15P; Intestinal bile acid-binding protein; I-BABP; FABP6; ILBP; ILLBP

CEP250

11190

Q9BV73

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human CEP250 recombinant protein (Position: Q563-K853) .

Isoform 1 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is xpressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. Isoform 2 is expressed in colorectal adenocarcinomas and their adjacent normal mucosa.

ELISA, Flow Cytometry, IHC, WB

Angiogenesis, Cancer, Cardiovascular, Cell Biology, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Binds to bile acids and is involved in enterohepatic bile acid metabolism. Required for efficient apical to basolateral transport of conjugated bile acids in ileal enterocytes. In vitro binds to bile acids in the order: deoxycholic acid > cholic acid > chenodeoxycholic acid and respective BA conjugation modifies affinities in the order taurine-conjugated > glycine-conjugated > unconjugated bile acids. Stimulates gastric acid and pepsinogen secretion. Essential for the survival of colon cancer cells to bile acid-induced apoptosis.

1. Bahe, S., Stierhof, Y.-D., Wilkinson, C. J., Leiss, F., Nigg, E. A. Rootletin forms centriole-associated filaments and functions in centrosome cohesion. J. Cell Biol. 171: 27-33, 2005. 2. de Castro-Miro, M., Tonda, R., Escudero-Ferruz, P., Andres, R., Mayor-Lorenzo, A., Castro, J., Ciccioli, M., Hidalgo, D. A., Rodriguez-Ezcurra, J. J., Farrando, J., Perez-Santonja, J. J., Cormand, B., Marfany, G., Gonzalez-Duarte, R. Novel candidate genes and a wide spectrum of structural and point mutations responsible for inherited retinal dystrophies revealed by exome sequencing. PLoS One 11: e0168966, 2016. Note: Electronic Article. 3. Fry, A. M., Mayor, T., Meraldi, P., Stierhof, Y.-D., Tanaka, K., Nigg, E. A. C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. J. Cell Biol. 141: 1563-1574, 1998.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

https://www.bosterbio.com/msds?sku=A07150-1

25503 MW

250 kDa

6

Fatty acid binding protein 6

Cytoplasm. Membrane. Peripheral membrane protein. Cytoplasmic side.

Metalloproteinase inhibitor 4

Rabbit IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.