Anti-CHD4 Antibody Picoband® 31.0550 Vervoeging

Chromodomain-helicase-DNA-binding protein 4 is an enzyme that in humans is encoded by the CHD4 gene. The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.

CHD4; Chromodomain-helicase-DNA-binding protein 4; CHD-4; EC 3.6.4.12; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta

CHD4

1108

Q14839

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the N-terminus of human CHD4, identical to the related mouse sequences.

Flow Cytometry

Immunogen affinity purified.

Liquid

1. Lan, X., Ren, R., Feng, R., Ly, L. C., Lan, Y., Zhang, Z., Aboreden, N., Qin, K., Horton, J. R., Grevet, J. D., Mayuranathan, T., Abdulmalik, O., Keller, C. A., Giardine, B., Hardison, R. C., Crossley, M., Weiss, M. J., Cheng, X., Cheng, X., Shi, J., Blobel, G. A. ZNF410 uniquely activates the NuRD component CHD4 to silence fetal hemoglobin expression. Molec. Cell 81: 239-254, 2021. 2. Le Gallo, M., O'Hara, A. J., Rudd, M. L., Urick, M. E., Hansen, N. F., O'Neil, N. J., Price, J. C., Zhang, S., England, B. M., Godwin, A. K., Sgroi, D. C., NIH Intramural Sequencing Center (NISC) Comparative Sequencing Program, Hieter, P., Mullikan, J. C., Merino, M. J., Bell, D. W. Exome sequencing of serous endometrial tumors identifies recurrent somatic mutations in chromatin-remodeling and ubiquitin ligase complex genes. Nature Genet. 44: 1310-1315, 2012. 3. Ostapcuk, V., Mohn, F., Carl, S. H., Basters, A., Hess, D., Iesmantavicius, V., Lampersberger, L., Flemr, M., Pandey, A., Thoma, N. H., Betschinger, J., Buhler, M. Activity-dependent neuroprotective protein recruits HP1 and CHD4 to control lineage-specifying genes. Nature 557: 739-743, 2018.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

218 kDa

6

Chromodomain helicase DNA binding protein 4

Chromodomain-helicase-DNA-binding protein 4

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.