Anti-CISH Antibody Picoband® fluoro488 Vervoeging

CISH (cytokine inducible SH2-containing protein), also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling (SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide (LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.

Cytokine-inducible SH2-containing protein; CIS; CIS-1; Protein G18; Suppressor of cytokine signaling; SOCS; CISH; G18

CISH

1154

Q9NSE2

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human CISH, identical to the related rat and mouse sequences.

Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. .

Flow Cytometry

Adapters, Cytoplasmic, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Signal Transduction, Transcription

Immunogen affinity purified.

Liquid

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate- recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity) . .

1. Hu, G., Zhou, R., Liu, J., Gong, A.-Y., Eischeid, A. N., Dittman, J. W., Chen, X.-M. MicroRNA-98 and let-7 confer cholangiocyte expression of cytokine-inducible Src homology 2-containing protein in response to microbial challenge. J. Immun. 183: 1617-1624, 2009. 2. Khor, C. C., Vannberg, F. O., Chapman, S. J., Guo, H., Wong, S. H., Walley, A. J., Vukcevic, D., Rautanen, A., Mills, T. C., Chang, K.-C., Kam, K.-M., Crampin, A. C., and 23 others. CISH and susceptibility to infectious diseases. New Eng. J. Med. 362: 2092-2101, 2010. 3. Uchida, K., Yoshimura, A., Inazawa, J., Yanagisawa, K., Osada, H., Masuda, A., Saito, T., Takahashi, T., Miyajima, A., Takahashi, T. Molecular cloning of CISH, chromosome assignment to 3p21.3, and analysis of expression in fetal and adult tissues. Cytogenet. Cell Genet. 78: 209-212, 1997.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

28663 MW

No cross reactivity with other proteins.

6

Cytokine-inducible SH2-containing protein

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Localizes on centrosomes in interphase cells and at each spindle pole in mitosis. Associates with both the pericentriolar material (PCM) and centrioles. Colocalized with SIRT2 at centrosome (By similarity) . Detected at the neurite hillock in developing neurons. .

Contains 1 SH2 domain.

Cytokine-inducible SH2-containing protein (CIS)

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.