Anti-CPSF6 Antibody Picoband® (monoklonale, 3F11E1)

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene. The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.

Coatomer subunit epsilon; Epsilon-coat protein; Epsilon-COP; COPE

CPSF6

11052

Q16630

Human, Mouse, Rat, Monkey

No cross-reactivity with other proteins.

E.coli-derived human CPSF6 recombinant protein (Position: R50-Q176) .

Clone: 3F11E1

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

Flow Cytometry

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Immunogen affinity purified.

Liquid

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.

1. Rasaiyaah, J., Tan, C. P., Fletcher, A. J., Price, A. J., Blondeau, C., Hilditch, L., Jacques, D. A., Selwood, D. L., James, L. C., Noursadeghi, M., Towers, G. J. HIV-1 evades innate immune recognition through specific cofactor recruitment. Nature 503: 402-405, 2013. 2. Ruegsegger, U., Blank, D., Keller, W. Human pre-mRNA cleavage factor I-m is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits. Molec. Cell 1: 243-253, 1998.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Coatomer protein complex subunit epsilon

Golgi apparatus membrane. Peripheral membrane protein. Cytoplasmic side. Cytoplasm. COPI-coated vesicle membrane.

Ras-related protein Rab-11B

Mouse IgG1

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.