Anti-CREB (Ser-271), Fosfospecifiek antilichaam

CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. CREB is expressed in various tissues, and has important gene-regulating roles in the nervous system. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. CREB is phosphorylated at other sites in response to calcium influx and DNA damage. The DNA-damage responsive nuclear kinase, HIPK2, can phosphorylate Ser-271 but not Ser-133 in CREB, and this phosphorylation activates CREB transactivation function. Mutation of Ser-271 to Glu-271 potentiates the CREB transactivation function. Thus, phosphorylation of Ser-271 may be the mode of activation for CREB-dependent transcription in response to genotoxic stress.

Our CREB (Ser-271) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat CREB (Ser-271) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Our CREB (Ser-271) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat CREB (Ser-271) and is antigen affinity purified. It is great for use in WB, ICC.

Cyclic AMP, CREB

CREB1

P16220

Ser-271

Chicken, Human, Mouse, and Rat

Phospho-CREB (Ser-271) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-271 in human CREB. This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB. The site has some homology to the conserved site in ATF1 (Ser-198) and CREM (Ser-287) .

CREB (Ser-271)

Unconjugated

ELISA, WB, ICC

Cell Signaling, Kinase Signaling, Epigenetics, Oncoproteins, Transcription Regulation

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

43

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, and rat PC12 cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

1:200

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_10837645