Anti-CRMP2 (Thr-555), Fosfospecifiek antilichaam

CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518, Thr-514, and Thr-509), and a priming site (Ser-522) . These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.

Our CRMP2 (Thr-555) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CRMP2 (Thr-555) and is antigen affinity purified. It is great for use in ELISA, WB.

Our CRMP2 (Thr-555) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CRMP2 (Thr-555) and is antigen affinity purified. It is great for use in WB.

DRP-2, Toad-64, CRMP-62

DPYSL2

Q16555

Thr-555

Human, Mouse, and Rat

Clone M539 was generated from a phospho-CRMP2 (Thr-555) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-555 in human CRMP2. This sequence is conserved in rat and mouse CRMP2 and the phospho-site is not conserved in other CRMP family members.

CRMP2 (Thr-555)

M539

Unconjugated

ELISA, WB

Neurodegenerative Disease, Alzheimer's Disease, Neurogenesis and Development

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

70

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:500

The antibody detects a 70 kDa* protein corresponding to CRMP2 on immunoblots of rat PC12 or mouse C2C12 cells treated with calyculin A. This reactivity is not observed after lambda phosphatase dephosphorylation of Thr-555.

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_3068402