Anti-Cyclin D2 Ccnd2 Antilichaam (Monoclonal, DCS-3)

Inaba et al. (1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2 (CCND2) gene ia assigned to 12p13.

Boster Bio Anti-Cyclin D2 Ccnd2 Antibody (Monoclonal, DCS-3) catalog # MA1034. Tested in ICC, WB applications. This antibody reacts with Human, Mouse.

G1/S-specific cyclin-D2; Vin-1 proto-oncogene; Ccnd2; Vin-1

CCND2

Human (894), Mouse (12444), Rat (64033)

Q04827

Human, Mouse

No cross-reactivity with other proteins

Recombinant human cyclin D2 protein.

Clone: DCS-3

ICC, WB

Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling

Ascites

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Lyophilized

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity) . .

1. Kozar, K.; Ciemerych, M. A.; Rebel, V. I.; Shigematsu, H.; Zagozdzon, A.; Sicinska, E.; Geng, Y.; Yu, Q.; Bhattacharya, S.; Bronson, R. T.; Akashi, K.; Sicinski, P. : Mouse development and cell proliferation in the absence of D-cyclins. Cell 118: 477-491, 2004. 2. Inaba, T.; Matsushime, H.; Valentine, M.; Roussel, M. F.; Sherr, C. J.; Look, A. T. : Genomic organization, chromosomal localization, and independent expression of human cyclin D genes. Genomics 13: 565-574, 1992. 3. Xiong, Y.; Menninger, J.; Beach, D.; Ward, D. C. : Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics 13: 575-584, 1992.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=MA1034

32826 MW

55 kDa

No cross reactivity with other proteins.

G1/S-specific cyclin-D2

Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .

Belongs to the cyclin family. Cyclin D subfamily.

G1/S-specific cyclin-D2

Mouse IgG2a

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.