Anti-Cytokeratin Peptide 18 Monoklonale Antilichaam (Kloon: CY-90)

Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

CK 18, CK18, CYK18, Cytokeratin endo B, K18

KRT18

3875

P05783

Human

The Human epidermal carcinoma A-431 and MCF-7 Human breast cancer cell lines.

Keratin, type I cytoskeletal 18

CY-90

WB, IHC-P, IHC-F

Ascites

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western blot : 0.5-1μg/ml; Immunohistochemistry (Paraffin-embedded Section) : 1-2μg/ml; Immunohistochemistry (Frozen Section) : 0.5-2μg/ml

Mouse IgG1