Anti-DNA-PKcs PRKDC Konijn Monoklonale Antilichaam

Boster Bio Anti-DNA-PKcs PRKDC Rabbit Monoclonal Antibody catalog # M00645. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs;2.7.11.1; DNPK1; p460; PRKDC; HYRC, HYRC1

PRKDC

5591

P78527

Human, Mouse, Rat

A synthesized peptide derived from human DNA-PKcs

Clone: EIA-16

Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

WB, IHC, ICC, IF

2339, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling

Affinity-chromatography

0.5mg/ml

Liquid

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V (D) J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V (D) J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) . The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an in machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M00645

469089 MW

85 kDa

DNA-dependent protein kinase catalytic subunit

Nucleus. Nucleus, nucleolus.

T-cell surface glycoprotein CD4

Rabbit IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.