Anti-DOK1 Konijn Monoklonale Antilichaam

Boster Bio Anti-DOK1 Rabbit Monoclonal Antibody catalog # M03039. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Ubiquitin-conjugating enzyme E2 R1;6.3.2.19; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1; CDC34; UBCH3, UBE2R1

DOK1

1796

Q99704

Human, Mouse, Rat

A synthesized peptide derived from human DOK1

Clone: 25D44

Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. .

WB, ICC, IF

Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Ubiquitin & Ubiquitin Like Modifiers

Affinity-chromatography

0.5mg/ml

Liquid

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF (FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF (SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes. .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M03039

26737 MW

65 kDa

Ubiquitin-conjugating enzyme E2 R1

Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

T-cell surface glycoprotein CD3 zeta chain

IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.