Anti-dok1 (Ser-450), fosforspecifiek antilichaam

Doks are a family of adaptor proteins that include six Dok proteins (Dok1 to Dok6), which have an N-terminal pleckstrin homology domain, a central phosphotyrosine binding domain, and a C-terminal region containing multiple tyrosine residues. When phosphorylated, these tyrosines can serve as docking sites for SH2 domain-containing proteins. Dok1 (p62dok) has been shown to bind Ras-GAP, Nck, and Csk. Several tyrosine phosphorylation sites have been identified for Dok1. One site, Tyr-362 (Tyr-361 mouse), is phosphorylated by c-Abl, is required for Nck binding, and may be critical for filopodia formation during fibroblast spreading on fibronectin. Alternatively, Dok1 activity is also regulated by serine phosphorylation. IκB Kinase β phosphorylates several serine sites including Ser-450 in vitro, and TNFα, IL-1, and radiation treatment lead to phosphorylation of Ser-443, Ser-446, and Ser-450 in vivo. Phosphorylation of these serine sites may be required for Dok-mediated inhibition of MAPK signaling and stimulation of cell motility.

Our Dok1 (Ser-450) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Dok1 (Ser-450) and is antigen affinity purified. It is great for use in ELISA, WB.

Our Dok1 (Ser-450) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Dok1 (Ser-450) and is antigen affinity purified. It is great for use in WB.

P62DOK

DOK1

Q99704

Ser-450

Human, Mouse, and Rat

Phospho-Dok1 (Ser-450) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 450 in human Dok1. This sequence is conserved in Dok1 from rat (Ser-449) and mouse (Ser-451) . The site is not conserved in other Dok family members.

Dok1 (Ser-450)

WB, E

Unconjugated

ELISA, WB

Cardiovascular, Cell Signaling, Oncoproteins, Scaffold Proteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

62

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

This antibody was cross-adsorbed to an unphosphorylated Dok1 (Ser-450) peptide before affinity purification using phospho-Dok1 (Ser-450) peptide. The purified antibody detects a band at 62 kDa* corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after lambda phosphatase treatment. Similar to Dok1 (Tyr-362) antibody, this antibody also detects an 80 kDa band that has not been identified.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2094938