Anti-Dopamine beta-hydroxylase (DBH) Antilichaam

DBH is an oxireductase belonging to the copper type II ascorbate-dependent monooxygenase family. DBH exists as a homotetramer composed of two non-covalently bound disulfide-linked dimers. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It binds 2 copper ions and 1 PQQ per subunit . Depending on the presence of a signal peptide, DBH can exist in both soluble and membrane-bound forms.

Our Anti-Dopamine beta-hydroxylase (DBH) rabbit polyclonal primary antibody detects bovine, guinea pig, human, and rat Dopamine beta-hydroxylase (DBH), and is whole serum. It is validated for use in IHC-Frozen, Neutralize, WB.

Our Anti-Dopamine beta-hydroxylase (DBH) rabbit polyclonal primary antibody detects bovine, guinea pig, human, and rat Dopamine beta-hydroxylase (DBH), and is whole serum. It is validated for use in IHC-Frozen, Neutralization, WB.

Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; Soluble dopamine beta-hydroxylase

P15101

Bovine, Guinea Pig, Human, and Rat

Native enzyme prepared from Bovine adrenal glands. Purity was greater than 95% by PAGE.

Dopamine beta-hydroxylase (DBH)

Unconjugated

IHC-Frozen, Neutralize, WB

Whole serum

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

1:500-1:2000

1:500-1:2000

This antiserum has been extensively characterised in several laboratories. It reacts with a single band on Western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme. This antibody is known to react with bovine, human, guinea pig and rat DBH.

Rabbit

Lyophilized

Mixed

Bovine