Anti-DPH5 Antibody Picoband® fluoro488 Vervoeging

Diphthine synthase is an enzyme that in humans is encoded by the DPH5 gene. This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene.

Solute carrier family 2, facilitated glucose transporter member 6; Glucose transporter type 6; GLUT-6; Glucose transporter type 9; GLUT-9; SLC2A6; GLUT9

DPH5

51611

Q9H2P9

Human

No cross-reactivity with other proteins.

E.coli-derived human DPH5 recombinant protein (Position: D11-H250) .

Highly expressed in brain, spleen and peripheral blood leukocytes.

Flow Cytometry

Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction

Immunogen affinity purified.

Liquid

Facilitative glucose transporter; binds cytochalasin B with low affinity.

1. Carette, J. E., Guimaraes, C. P., Varadarajan, M., Park, A. S., Wuethrich, I., Godarova, A., Kotecki, M., Cochran, B. H., Spooner, E., Ploegh, H. L., Brummelkamp, T. R. Haploid genetic screens in human cells identify host factors used by pathogens. Science 326: 1231-1235, 2009. 2. Liu, S., Milne, G. T., Kuremsky, J. G., Fink, G. R., Leppla, S. H. Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Molec. Cell. Biol. 24: 9487-9497, 2004. 3. Zhang, Q.-H., Ye, M., Wu, X.-Y., Ren, S.-X., Zhao, M., Zhao, C.-J., Fu, G., Shen, Y., Fan, H.-Y., Lu, G., Zhong, M., Xu, X.-R., and 9 others. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res. 10: 1546-1560, 2000.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Solute carrier family 2 member 6

Cell membrane. Multi-pass membrane protein.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.