Anti-DUT Antibody Picoband® fluoro488 Vervoeging

Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35% identity with the E.coli dUTPase and 53% identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92% identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTPase;3.6.1.23; dUTP pyrophosphatase; DUT

DUT

1854

P33316

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human DUT, different from the related rat and mouse sequences by two amino acids.

Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level) . Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level) . .

Flow Cytometry

DNA/Nucleotides, DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Immunogen affinity purified.

Liquid

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. .

1. Studebaker AW, Lafuse WP, Kloesel R, Williams MV.Modulation of human dUTPase using small interfering RNA. Biochem Biophys Res Commun. 2005 Feb 4; 327 (1) :306-10. 2. McIntosh EM, Ager DD, Gadsden MH, Haynes RH.Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc Natl Acad Sci U S A. 1992 Sep 1; 89 (17) :8020-4. 3. Chu R, Lin Y, Rao MS, Reddy JK.Cloning and identification of rat deoxyuridine triphosphatase as an inhibitor of peroxisome proliferator-activated receptor alpha. J Biol Chem. 1996 Nov 1; 271 (44) :27670-6. 4. Ladner RD, Caradonna SJ.The human dUTPase gene encodes both nuclear and mitochondrial isoforms.Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. J Biol Chem. 1997 Jul 25; 272 (30) :19072-80. 5. Ladner RD.The role of dUTPase and uracil-DNA repair in cancer chemotherapy. Curr Protein Pept Sci. 2001 Dec; 2 (4) :361-70.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

27 kDa

No cross reactivity with other proteins.

6

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Isoform 2: Nucleus.

Belongs to the dUTPase family.

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.