Anti-E-Cadherine (Cytoplasmatisch) Antilichaam

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Our E-Cadherin (Cytoplasmic) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat E-Cadherin (Cytoplasmic) and is protein a purified. It is great for use in ELISA, WB, ICC, IHC, IP.

Our E-Cadherin (Cytoplasmic) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat E-Cadherin (Cytoplasmic) and is Protein A purified. It is great for use in WB, ICC, IHC, IP.

Uvomorulin, Cadherin-1, CTF1, CTF2, CTF3, CD324, Epithelial Cadherin

CDH1

P09803

Human, Mouse, and Rat

Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the cytoplasmic region. This sequence is highly conserved in human and rat E-cadherin.

E-Cadherin (Cytoplasmic)

M168

Unconjugated

ELISA, WB, ICC, IHC, IP

Cell Adhesion Molecules, Cell Markers, Extracellular Matrix Proteins, Oncoproteins

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

GOmAb

120

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:50

1:1000

1:100

This E-cadherin antibody detects a 120 kDa* protein in human A431 cells, and does not cross-react with VE-cadherin or N-cadherin.

1:250

Western blots performed on each lot.

Mouse

Liquid

IgG1

Mouse

Quality Control: WB

AB_2076812