Anti-EB3-antilichaam

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs) . These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (α) and short (β) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP) . The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.

Our EB3 primary antibody from PhosphoSolutions is rat monoclonal. It detects human, mouse, and rat EB3 and is protein g purified. It is great for use in ELISA, WB, ICC.

Our EB3 primary antibody from PhosphoSolutions is rat monoclonal. It detects human, mouse, and rat EB3 and is Protein G purified. It is great for use in WB, ICC.

Microtubule RP/EB, MAPRE3, APC, EB3, End-binding. RP3

MAPRE3

Q9UPY8

Human, Mouse, and Rat

Clone KT53 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3.

EB3

KT53

Unconjugated

ELISA, WB, ICC

Cell Markers, Cytoskeletal Markers, Cell Signaling, Cytoskeletal Regulation

Protein G Purified

PBS + 0.05% NaN3

32

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Protein G purified culture supernatant.

1:1000

The antibody detects a 32 kDa* prtoein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells.

1:100

Western blots performed on each lot.

Rat

Liquid

IgG2a

Human

Quality Control: WB

AB_3068427