Anti-EB3 (centraal gebied) Antilichaam

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs) . These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (α) and short (β) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP) . The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.

Our EB3 (central region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EB3 (central region) and is antigen affinity purified. It is great for use in ELISA, WB.

Our EB3 (central region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EB3 (central region) and is antigen affinity purified. It is great for use in WB.

Microtubule RP/EB, MAPRE3, APC, EB3, End-binding. RP3

MAPRE3

Q9UPY8

Human, Mouse, and Rat

EB3 synthetic peptide (coupled to KLH) corresponding to amino acid residues from the central region of human EB3. This peptide sequence is highly conserved in mouse and rat EB3, and has low homology to other EB family members.

EB3 (central region)

Unconjugated

ELISA, WB

Cell Markers, Cytoskeletal Markers, Cell Signaling, Cytoskeletal Regulation

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

32

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects a 32 kDa* protein corresponding to the apparent molecular mass of EB3 on SDS-PAGE immunoblots of mouse brain and human recombinant full length EB3 protein.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_3068435