Anti-EB3 (Ser-176), Fosfospecifiek antilichaam

Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs) . These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3) . EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 protein stability during mitosis.

Our EB3 (Ser-176) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EB3 (Ser-176) and is antigen affinity purified. It is great for use in ELISA, WB.

Our EB3 (Ser-176) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EB3 (Ser-176) and is antigen affinity purified. It is great for use in WB.

Microtubule RP/EB, MAPRE3, APC, EB3, End-binding. RP3

MAPRE3

Q9UPY8

Ser-176

Human, Mouse, and Rat

Phospho-EB3 (Ser-176) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 176 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3. This site is conserved in EB2 but the neighboring amino acids are not well conserved.

EB3 (Ser-176)

Unconjugated

ELISA, WB

Cell Signaling, Cytoskeletal Regulation

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

32

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:500

This antibody was cross-absorbed to unphospho-EB3 (Ser-176), before affinity purification using phospho-EB3 (Ser-176) peptide (without carrier) . The antibody detects a 32 kDa* protein corresponding to the apparent molecular mass of phosphorylated EB3 on SDS-PAGE immunoblots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_3068436