Anti-EGFR (Ser-1142), fosforspecifiek antilichaam

The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173) . These sites are important for downstream signaling and rapid internalization. In addition, EGFR activation leads to c-Src mediated phosphorylation of Tyr-845 and Tyr-1101. The former site is required for mitogenic responses to EGFR activation, while the latter may be an SH2 binding site. Phosphorylation of EGFR on serine and threonine residues is thought to represent a mechanism for regulation of receptor kinase activity and internalization. These sites include a PKC site (Thr-654), CAMKII sites (Ser-1046, 1047, 1057, and 1142), and constitutively phosphorylated sites (Ser-967 and Ser-1002) . Thus, the regulation of EGFR activity involves a complex series of phosphorylation events at multiple sites throughout the intracellular portion of the receptor.

Our EGFR (Ser-1142) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EGFR (Ser-1142) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Our EGFR (Ser-1142) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EGFR (Ser-1142) and is antigen affinity purified. It is great for use in WB, ICC.

EGF, Epidermal, ErbB

EGFR

P00533

Ser-1142

Human, Mouse, and Rat

Phospho-EGFR (Ser-1142) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 1142 of human EGFR (ErbB-1) . This human EGFR sequence has high homology with rat and mouse EGFR, and is not conserved in other ErbB family members.

EGFR (Ser-1142)

Unconjugated

ELISA, WB, ICC

Cell Adhesion Molecules, Cell Signaling, Growth Factor Signaling, Kinase Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

180

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

This antibody was cross-adsorbed to unphosphorylated EGFR (Ser-1142) coupled to agarose then affinity purified using phospho-EGFR (Ser-1142) peptide (without carrier) . The antibody detects a 180 kDa* protein on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A, and does not detect this band after lambda phosphatase treatment.

1:200

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2096432