Anti-EIF2S1 Antibody Picoband® fluoro647 Vervoeging

EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz (2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence (GRCh37) . Ernst et al. (1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al. (1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.

Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2S1; EIF2A

EIF2S1

1965

P05198

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1.

Flow Cytometry

DNA/RNA, Epigenetics and Nuclear Signaling, RNA Processing, Translation

Immunogen affinity purified.

Liquid

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

1. Ernst, H., Duncan, R. F., Hershey, J. W. B. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2: characterization of the protein and its messenger RNA. J. Biol. Chem. 262: 1206-1212, 1987. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/28/2010. 3. Jacob, W. F., Silverman, T. A., Cohen, R. B., Safer, B. Identification and characterization of a novel transcription factor participating in the expression of eukaryotic initiation factor 2 alpha. J. Biol. Chem. 264: 20372-20384, 1989.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

36112 MW

No cross reactivity with other proteins.

6

Eukaryotic translation initiation factor 2 subunit 1

Cytoplasmic granule . The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity) . .

Belongs to the eIF-2-alpha family.

Eukaryotic translation initiation factor 2 subunit 1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.