Anti-eIF3B Antibody Picoband®

EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B), also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al. (1997) identified a cDNA encoding a human Prt1 homolog. Asano et al. (1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of 2 proteins, p116 (PRT1) and an unrelated 110-kD protein. Chaudhuri et al. (1997) isolated cDNAs encoding PRT1, which they called p110 based on the size of the corresponding component of rabbit eIF3.

Boster Bio Anti-eIF3B Antibody catalog # PA2030. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Eukaryotic translation initiation factor 3 subunit B ; eIF3b ; Eukaryotic translation initiation factor 3 subunit 9 ; Prt1 homolog; hPrt1; eIF-3-eta ; eIF3 p110 ; eIF3 p116; EIF3B ; EIF3S9

EIF3B

8662

P55884

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human eIF3B, different from the related rat and mouse sequences by one amino acid.

WB

DNA/RNA, Epigenetics and Nuclear Signaling, Translation

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. .

1. Asano, K., Kinzy, T. G., Merrick, W. C., Hershey, J. W. B. Conservation and diversity of eukaryotic translation initiation factor eIF3. J. Biol. Chem. 272: 1101-1109, 1997. 2. Chaudhuri, J., Chakrabarti, A., Maitra, U. Biochemical characterization of mammalian translation initiation factor 3 (eIF3) : molecular cloning reveals that p110 subunit is the mammalian homologue of saccharomyces cerevisiae protein Prt1. J. Biol. Chem. 272: 30975-30983, 1997. 3. Methot, N., Rom, E., Olsen, H., Sonenberg, N. The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170. J. Biol. Chem. 272: 1110-1116, 1997.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA2030

92482 MW

115 kDa

No cross reactivity with other proteins.

Eukaryotic translation initiation factor 3 subunit B

Cytoplasm .

Belongs to the eIF-3 subunit B family.

Eukaryotic translation initiation factor 3 subunit B

Rabbit IgG

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.