Anti-EIF4B Antibody Picoband® fluoro594 Vervoeging

Eukaryotic translation initiation factor 4B is a protein that in humans is encoded by the EIF4B gene. Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly- (A) binding protein, increasing its poly- (A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens, and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.

Complement component C7; C7

EIF4B

1975

P23588

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human EIF4B recombinant protein (Position: M1-E611) .

Widely expressed.

Flow Cytometry

Angiogenesis, Cancer, Cardiovascular, Developmental Biology, Embryogenesis, Growth Factors, Growth Factors/Hormones, Lineage Specification, Mesenchymal Stem Cells, Signal Transduction, Stem Cells

Immunogen affinity purified.

Liquid

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.

1. Garcia-Garcia, C., Frieda, K. L., Feoktistova, K., Fraser, C. S., Block, S. M. Factor-dependent processivity in human eIF4A DEAD-box helicase. Science 348: 1486-1488, 2015. 2. Methot, N., Pause, A., Hershey, J. W., Sonenberg, N. The translation initiation factor eIF-4B contains an RNA-binding region that is distinct and independent from its ribonucleoprotein consensus sequence. Molec. Cell. Biol. 14: 2307-2316, 1994. 3. Methot, N., Song, M. S., Sonenberg, N. A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3. Molec. Cell. Biol. 16: 5328-5334, 1996.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

55914 MW

6

Complement C7

Secreted.

Protein argonaute-1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.