Anti-EpCAM Antilichaam / Extracellulair domein

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

P16422

Human, Dog, Cat

A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the anti-EpCAM antibody.

EGP40/1372

IHC-P

Protein G affinity chromatography

Purified

This anti-EpCAM antibody is available for research use only.

Store the anti-EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Titering of the anti-EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Cell surface, cytoplasmic

IHC testing of FFPE human hepatocellular carcinoma and anti-EpCAM antibody (clone EGP40/1372) . Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.