Anti-EphA4 (C-terminaal gebied) Antilichaam

The Eph family of Receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. Eph receptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. The EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. EphA4 signaling pathways require its kinase activity and involve binding and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs) . EphA4 activation leads autophosphorylation of Tyr-596 and Tyr-602, and the conserved sites in EphA2 are required for binding to the GEFs, Vav2 and Vav3, and ephrin-induced cell migration. The Tyr-779 site in the kinase domain is also phosphorylated in vivo and may regulate kinase activity. Activated EphA4 leads to Src kinase phosphorylation of the GEF, ephexin-1, and this activates RhoA. Thus, EphA4 signaling involves complex tyrosine phosphorylation in its cytoplasmic region along with interaction with several GEFs.

Our EphA4 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EphA4 (C-terminal region) and is antigen affinity purified. It is great for use in ELISA, WB.

Our EphA4 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EphA4 (C-terminal region) and is antigen affinity purified. It is great for use in WB.

SEK, Eph

EPHA4

P54764

Human, Mouse, and Rat

EphA4 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human EphA4. This sequence has significant homology to the conserved site in rat and mouse EphA4, and has low homology to other EphA and EphB family members.

EphA4 (C-terminal region)

Unconjugated

ELISA, WB

Cardiovascular, Cell Signaling, Kinase Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

95/120

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects 95 and 120 kDa* bands corresponding to EphA4 in Western blots of HUVEC cells and mouse brain tissue.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_1268960