Anti-ERp57 Antibody Picoband® (monoklonale, 7E5)

PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme. It is mapped on 15q15.3. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; K8; Type-II keratin Kb8; KRT8; CYK8

PDIA3

2923

P30101

Human

No cross-reactivity with other proteins.

A synthetic peptide corresponding to a sequence at the C-terminus of human ERp57, different from the related mouse and rat sequences by two amino acids.

Clone: 7E5

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Flow Cytometry

Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Keratin, Signal Transduction

Immunogen affinity purified.

Liquid

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

1. Entrez Gene: PDIA3 protein disulfide isomerase family A, member 3. 2. Ellerman, D. A., Myles, D. G., Primakoff, P. A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57. Dev. Cell 10: 831-837, 2006 3. Garbi, N., Tanaka, S., Momburg, F., Hammerling, G. J. Impaired assembly of the major histocompatibility complex class I peptide-loading complex in mice deficient in the oxidoreductase ERp57. Nature Immun. 7: 93-102, 2006.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Keratin 8

Nucleoplasm. Nucleus matrix. Cytoplasm.

CD79b molecule

Mouse IgG1

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.