Anti-ERp57/PDIA3 Antibody Picoband® fluoro550 Vervoeging

PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme. It is mapped on 15q15.3. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Protein disulfide-isomerase A3;5.3.4.1;58 kDa glucose-regulated protein;58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60; PDIA3; ERP57, ERP60, GRP58

PDIA3

2923

P30101

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human ERp57, identical to the related rat and mouse sequences.

Detected in the flagellum and head region of spermatozoa (at protein level) . .

Flow Cytometry

ER Proteins, Protein Trafficking, Signal Transduction

Immunogen affinity purified.

Liquid

1. Entrez Gene: PDIA3 protein disulfide isomerase family A, member 3. 2. Ellerman, D. A., Myles, D. G., Primakoff, P. A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57. Dev. Cell 10: 831-837, 2006 3. Garbi, N., Tanaka, S., Momburg, F., Hammerling, G. J. Impaired assembly of the major histocompatibility complex class I peptide-loading complex in mice deficient in the oxidoreductase ERp57. Nature Immun. 7: 93-102, 2006.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

56782 MW

No cross reactivity with other proteins.

6

Protein disulfide-isomerase A3

Endoplasmic reticulum. Endoplasmic reticulum lumen . Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Belongs to the protein disulfide isomerase family.

Protein disulfide-isomerase A3

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.