Anti-Estrogeen receptor α (C-terminus) Antilichaam

Estrogen receptor α (ERα) is a member of the steroid receptor superfamily and its structure includes an N-terminal ligand-independent transactivation domain (AF-1), a highly conserved DNA binding domain, and a C-terminal ligand-dependent transactivation domain (AF-2) . AF-1 and AF-2 activate transcription independently and synergistically, and act in a promoter- and cell-specific manner. Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity. Ser-104, Ser-106, Ser-118, and Ser-167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. In addition to these sites, phosphorylation of Tyr-537 has been implicated in maximal hormone binding, dimerization, and transcriptional activity. Tyr-537, located in the AF-2 domain, is phosphorylated by c-Src leading to nuclear export of ERα and degradation. Thus, a variety of phosphorylation events control ERα activity.

Our Estrogen Receptor α (C-terminus) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat Estrogen Receptor α (C-terminus) and is antigen affinity purified. It is great for use in ELISA, WB, ICC, IP.

Our Estrogen Receptor α (C-terminus) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat Estrogen Receptor α (C-terminus) and is antigen affinity purified. It is great for use in WB, ICC, IP.

ESR, ESR1, ESRA, Estradiol receptor, Eralpha, ER

ESR1

P03372

Human, Mouse, and Rat

ERα (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminus of human ERα. This sequence is well conserved in rat and mouse ERα, but is not found in ERβ

Estrogen Receptor α (C-terminus)

Unconjugated

ELISA, WB, ICC, IP

Nuclear Hormone Receptors, Cell Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

35-66

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:500

1:50

This antibody detects several forms of ERα ranging from 66 to 35 kDa* on SDS-PAGE immunoblots of MCF-7 cells treated with pervanadate, and MDA-MB-231 cells.

1:100

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_3068437