Anti-Fat Free VPS51 Antilichaam

Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN) . Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.

Boster Bio Anti-Fat Free VPS51 Antibody (Catalog # A08518) . Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

FFR, ANG2, ANG3, C11orf2, C11orf3, FFR, PP5382, Vacuolar protein sorting-associated protein 51 homolog, Another new gene 2 protein

C11ORF2

Q9UID3

Human, Mouse, Rat

At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.

Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free. The immunogen is located within amino acids 620 - 670 of Fat Free.

ELISA, WB

Homeostasis

Fat Free Antibody is affinity chromatography purified via peptide column.

1 mg/mL

Liquid

Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN) . The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN (PubMed:20685960) . Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane (PubMed:25799061) .

Fat Free antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

https://www.bosterbio.com/msds?sku=A08518

86042 MW

Chromosome 11 open reading frame 2

Golgi apparatus, trans-Golgi network

Bovine, Chicken

Chromosome 11 open reading frame 2

IgG

Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.