Anti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) 310647 Vervoeging

Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; K8; Type-II keratin Kb8; KRT8; CYK8

FEN1

2237

P39748

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human FEN1 recombinant protein (Position: Q4-E300) .

Clone: 6F3F2

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Flow Cytometry

Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Keratin, Signal Transduction

Immunogen affinity purified.

Liquid

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

1. Hiraoka LR, Harrington JJ, Gerhard DS, Lieber MR, Hsieh CL (Jul 1995) . Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human. Genomics. 25 (1) : 220–5. 2. Dianova II, Bohr VA, Dianov GL (Oct 2001) . Interaction of human AP endonuclease 1 with flap endonuclease 1 and proliferating cell nuclear antigen involved in long-patch base excision repair. Biochemistry. 40 (42) : 12639–44. 3. Sharma S, Sommers JA, Wu L, Bohr VA, Hickson ID, Brosh RM (Mar 2004) . Stimulation of flap endonuclease-1 by the Bloom's syndrome protein. J. Biol. Chem. 279 (11) : 9847–56.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Keratin 8

Nucleoplasm. Nucleus matrix. Cytoplasm.

CD79b molecule

Mouse IgG1

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.