Anti-FHOD1 Antilichaam

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. Several mammalian formins, including mDia1, FRL, and formin homology domain protein 1 (FHOD1) are inhibited through an intramolecular interaction between the C-terminal Dia autoregulatory domain (DAD) and its recognition region at the N-terminus. In FHOD1, this autoinhibitory interaction is disrupted through phosphorylation of Ser-1131, Ser-1137, and Thr-1141 by ROCK. Subsequent FHOD1 activation leads to stress fiber formation. In endothelial cells, thrombin activates this ROCK pathway, leading to FHOD1-mediated stress fiber formation.

Our FHOD1 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human FHOD1 and is protein a purified. It is great for use in ELISA, WB, IHC.

Our FHOD1 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human FHOD1 and is Protein A purified. It is great for use in WB, IHC.

FHOS, formin

FHOD1

Q9Y613

Human

FHOD1 recombinant protein that included the full length human FHOD1 sequence. This sequence has 50% homology to human FHOD3, and low homology to other formin family members.

FHOD1

M352

Unconjugated

ELISA, WB, IHC

Cell Signaling, Cytoskeletal Regulation

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

140

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:50

1:250

The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of FHOD1 on SDS-PAGE immunoblots of human K562 and Jurkat, and has weak reactivity to FHOD1 in mouse C2C12.

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_2104508