Anti-GCC1 Antibody Picoband® fluoro488 Vervoeging

GRIP and coiled-coil domain-containing protein 1 is a protein that in humans is encoded by the GCC1 gene. The protein encoded by this gene is a peripheral membrane protein. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. It may play a role in the organization of trans-Golgi network subcompartment involved with membrane transport.

Negative elongation factor B; NELF-B; Cofactor of BRCA1; NELFB; COBRA1; KIAA1182

GCC1

79571

Q96CN9

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human GCC1 recombinant protein (Position: E22-L763) .

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Flow Cytometry

Epigenetics and Nuclear Signaling, Transcription

Immunogen affinity purified.

Liquid

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.

1. Kjer-Nielsen, L., Teasdale, R. D., van Vliet, C., Gleeson, P. A. A novel Golgi-localisation domain shared by a class of coiled-coil peripheral membrane proteins. Curr. Biol. 9: 385-388, 1999. 2. Luke, M. R., Kjer-Nielsen, L., Brown, D. L., Stow, J. L., Gleeson, P. A. GRIP domain-mediated targeting of two new coiled-coil proteins, GCC88 and GCC185, to subcompartments of the trans-Golgi network. J. Biol. Chem. 278: 4216-4226, 2003. 3. Wong, M., Munro, S. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science 346: 1256898, 2014. Note: Electronic Article.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Negative elongation factor complex member B

Nucleus.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.