Anti-GCN2/EIF2AK4 Antibody Picoband® fluoro647 Vervoeging

EIF2AK4 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4), also called GCN2, is an enzyme that in humans is encoded by the EIF2AK4 gene. EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. Hartz (2005) mapped the EIF2AK4 gene to chromosome 15q15.1 based on an alignment of the EIF2AK4 sequence with the genomic sequence. Berlanga et al. (1999) demonstrated that Gcn2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro. Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse Eif2ak4. Costa-Mattioli et al. (2005) reported a unique feature of hippocampal slices from Gcn2-null mice: in CA1, a single 100-Hz train induced a strong and sustained long-term potentiation (late LTP or L-LTP), which was dependent on transcription and translation.

Eukaryotic translation initiation factor 2-alpha kinase 4;2.7.11.1; GCN2-like protein; mGCN2; Eif2ak4; Gcn2, Kiaa1338

EIF2AK4

27103

Q9QZ05

Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of mouse GCN2, different from the related rat sequence by one amino acid.

Expressed in liver (at protein level) . Widely expressed, with the highest levels in the liver and the brain. Isoform 4 is expressed in brain followed by testis and liver and isoform 3 is expressed only in brain. .

Flow Cytometry

DNA/RNA, Epigenetics and Nuclear Signaling, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Translation

Immunogen affinity purified.

Liquid

Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.

1. Berlanga, J. J., Santoyo, J., de Haro, C. Characterization of a mammalian homolog of the GCN2 eukaryotic initiation factor 2-alpha kinase. Europ. J. Biochem. 265: 754-762, 1999. 2. Costa-Mattioli, M., Gobert, D., Harding, H., Herdy, B., Azzi, M., Bruno, M., Bidinosti, M., Ben Mamou, C., Marcinkiewicz, E., Yoshida, M., Imataka, H., Cuello, A. C., Seidah, N., Sossin, W., Lacaille, J.-C., Ron, D., Nader, K., Sonenberg, N. Translational control of hippocampal synaptic plasticity and memory by the elF2-alpha kinase GCN2. Nature 436: 1166-1170, 2005. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 3/28/2005.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

186487 MW

No cross reactivity with other proteins.

6

Eukaryotic translation initiation factor 2-alpha kinase 4

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.

EIF-2-alpha kinase GCN2

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.