Anti-Gelsolin/GSN Antibody Picoband®

Gelsolin also known as GNS is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. Gelsolin is also known as brevin, or actin-depolymerizing factor; it is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.

Boster Bio Anti-Gelsolin/GSN Antibody catalog # PA2109. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Gelsolin; AGEL; Actin-depolymerizing factor; ADF; Brevin; GSN

GSN

2934

P06396

Human, Monkey, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.

Flow Cytometry, IF, IHC, ICC, WB

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping) . It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. .

1. Kamada, S., Kusano, H., Fujita, H., Ohtsu, M., Koya, R. C., Kuzumaki, N., Tsujimoto, Y. A cloning method for caspase substrates that uses the yeast two-hybrid system: cloning of the antiapoptotic gene gelsolin. Proc. Nat. Acad. Sci. 95: 8532-8537, 1998. 2. Kim, J., Lee, J. E., Heynen-Genel, S., Suyama, E., Ono, K., Lee, K., Ideker, T., Aza-Blanc, P., Gleeson, J. G. Functional genomic screen for modulators of ciliogenesis and cilium length. Nature 464: 1048-1051, 2010. 3. Vasconcellos, C. A., Allen, P. G., Wohl, M. E., Drazen, J. M., Janmey, P. A., Stossel, T. P. Reduction in viscosity of cystic fibrosis sputum in vitro by gelsolin. Science 263: 969-971, 1994.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA2109

85698 MW

90 kDa

No cross reactivity with other proteins.

6

Gelsolin

Isoform 2: Cytoplasm, cytoskeleton.

Belongs to the villin/gelsolin family.

Gelsolin

Rabbit IgG

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.