Anti-GFP (N86/38) Recombinant kip Chimeric mAb FL490 Conjugate

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Our chicken Anti-GFP recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Our chicken Anti-GFP FL490 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects n/a GFP and is purified by affinity chromatography. It works in IHC, ICC.

Green fluorescent protein

GFP

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

GFP

N86/38

FL490 Ex: 491 nm, Em: 515 nm

IHC, ICC

Quality Control: ICC/Anti-Tag Antibodies/

Primary Antibodies

0.5 mg/mL

Purified by affinity chromatography

Liquid

7.4

27 kDa

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipped on ice packs

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

1:500-1:1000

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively) Does not cross-react with Red fluorescent protein (dsRed)

1:500-1:1000

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

P42212

IgY

Jellyfish