Anti-GFP (N86/38) Recombinant kip Chimeric mAb FL650 Conjugaat

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Our chicken Anti-GFP recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Our chicken Anti-GFP FL650 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects n/a GFP and is purified by affinity chromatography. It works in IHC, ICC.

Green fluorescent protein

GFP

P42212

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

GFP

N86/38

FL650 Ex: 655 nm, Em: 676 nm

Primary Antibodies

IHC, ICC

Anti-Tag Antibodies

0.5 mg/mL

Purified by affinity chromatography

7.4

27 kDa

Shipped on ice packs

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

1:500-1:1000

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively) Does not cross-react with Red fluorescent protein (dsRed)

1:500-1:1000

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Aves Labs

Liquid

IgY

Jellyfish

1