Anti-GLI1 Antibody Picoband® 0,00647 Vervoeging

GLI1, also known as GLI, is a protein originally isolated in human glioblastoma. The protein is encoded by GLI1 gene. It is mapped to 12q13.3. This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Beside, various upstream patterning signals may be integrated by the Gli proteins to a coherent programme of neurogenesis.

GLI1

2735

P08151

Human, Mouse, Rat

E.coli-derived human GLI1 recombinant protein (Position: Y800-Q1057) . Human GLI1 shares 79.8% amino acid (aa) sequence identity with mouse GLI1.

Flow Cytometry

Immunogen affinity purified.

Liquid

1. Kinzler KW, ; Bigner SH; Bigner DD; Trent JM; Law ML; O'Brien SJ; Wong AJ; Vogelstein B. (April 1987) . Identification of an amplified, highly expressed gene in a human glioma. Science 236 (4797) : 70–3. 2. Ruiz i Altaba A. (June 1999) . Gli proteins encode context-dependent positive and negative functions: implications for development and disease.. Development 126 (14) : 3205–16. 3. Sasaki H, ; Hui C; Nakafuku M; Kondoh H. (April 1997) . A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development 124 (7) : 1313–22.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.